Recombinant Human DnaJ
Recombinant Human DnaJ
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Hsp40
Gene Name
DNAJB1
UniProt
P25685
Cellular Locus
Cytoplasm , Nucleus , Nucleus, nucleolus , Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
Reactivity
Human
Target Antigen
DnaJ homolog subfamily B member 1
Target
DnaJ
Type
Protein
Applications
WB
Concentration
Lot Specific
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Form
Liquid
Buffer
20 mM Tris
Function
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and rPubMed:24318877, PubMed:9499401}.
Storage Conditions
Although stable at 4°C for 4 weeks, product should be stored at or below -20°C. For long term storage, addition of a carrier protein (0.1% HSA or BSA) is recommended.
Product Datasheet
https://www.qedbio.com/wp-content/uploads/pdf/11111P.pdf
Specificity
DnaJ
Formulation
Sterile-filtered colorless solution contains 20mM Tris buffer, pH 8.0, 2mM DTT, 0.2mM PMSF, and 10% glycerol.
Buffer pH
pH 8.0
Target Background
Human Hsp40 is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in protein folding, transport, and assembly. Hsp40 protein contains the J-domain which controls interactions with the ATPase domain of DnaK.
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