Anti-HGAL Antibody

Mouse Monoclonal Antibody specific to HGAL

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Germinal center B-cell-expressed transcript 2 protein, Germinal center-associated lymphoma protein, hGAL

Gene Name

GCSAM

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=GCSAM">GCSAM</a>

UniProt

Q8N6F7

Cellular Locus

Cytoplasm. Cell membrane. Note=It relocalizes from the cytoplasm to podosome-like structures upon cell treatment with IL6.

Host

Mouse

Reactivity

Human

Immunogen

GST-HGAL fusion protein (Accession no. AF521911)

Target Antigen

Germinal center-associated signaling and motility protein

Target

HGAL

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. {PubMed:17823310, PubMed:20844236, PubMed:23299888}.

Additionnal Information

Immunohistochemistry: use at 1-10ug/ml on paraffin-embedded tissues with heat-induced antigen retrieval in citrate buffer (10mM, pH 6.0, 10 minutes). Immunoblotting: use at 1-10ug/mlHGAL mRNA and protein expression in lysates of indicated cell lines. Actin served as a loading control. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human HGAL.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

The human germinal-center-associated lymphoma (HGAL) gene was originally identified from an expressed sequence tag that was associated with improved survival in patients with diffuse large B-cell lymphoma (DLBCL). Cloning and characterization of HGAL indicated that it is induced by IL-4. HGAL is a cytoplasmic protein and has been demonstrated in follicular lymphoma, Burkitt lymphoma, lymphocyte-predominant Hodgkin lymphoma, and a subset of DLBCL. HGAL expression is an important predictor of overall survival in patients with germinal center- derived tumors. HGAL protein is also found in germinal centers of normal tonsils and lymph nodes.