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Benzylaminopurine (6-BAP)

Benzylaminopurine (BAP) Powder is a plant growth regulator commonly used in plant tissue culture applications to promote cell division and shoot proliferation.

Product Specifications

CAS Number

1214-39-7

Synonyms

Benzyl Adenine, BAP or BA

HS Code

2933.99

Applications

Micropropagation of various plant species Somatic embryogenesis Stimulating seed germination Shoot development in vitro Breaking bud dormancy

Assay Protocol

Follow the given steps to prepare the tissue culture media using PCT’s BAP powder: Prepare liquid tissue culture media using PCT’s MS or DKW media, or any other media based on your plant. Add sugar based on the quantity of media you’re making (generally for 1-liter media 30 grams of sugar is added), 1 ml/L PPM, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar. Then, add the desired quantity of the BAP from the prepared BAP stock solution. Autoclave the medium for 20 minutes at 15 psi and 121°C.

Solubility

Soluble in KOH or NaOH

Molecular Formula

C12H11N5

Molecular Weight

225.25 g/mol

Features & Benefits

Easy to use Influences plant growth and development, sets blossoms, and stimulates fruit richness by stimulating cell division Use to supplement tissue culture media, such as MS media Stimulates seed germination. Used for shoot development in vitro. Also, supplement Gamborg’s media and Chu’s N6 media.

Storage Temperature

2-8°C

Shelf Life

3 years from the manufacturing date

Appearance

White to pale yellow powder

Suitability

Passes for Tissue Culture

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