Goat anti-GAPDH Polyclonal antibody

Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase) conjugated to DyLight® 755. GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects a band of 37 kDa by Western blot.

Product Name Alternative

glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.

UNSPSC Description

Glyceraldehyde-3-phosphate dehydrogenase

Volume

100 µL

Gene ID

ENSG00000111640

Accession Number

ENSG00000111640

Host

Goat

Antigen Species

Human

Reactivity

Reacts against human, rat, mouse, zebrafish, canine and monkey proteins.

Immunogen

Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-GAPDH, DyLight®755

Clonality

Polyclonal

Isotype

IgG

Conjugation

DyLight®755

Type

Primary

Sequence

SVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE

Applications

WB, IF, IHC-F

Purification

Epitope affinity purified

Concentration

2.5 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:250, IHC-F:1:200-1:1,000

Form

Polyclonal antibody supplied as a 100 µl (2.5 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.