Nitrocefin (Standard)

Nitrocefin (Standard) is the analytical standard of Nitrocefin. This product is intended for research and analytical applications. Nitrocefin is a chromogenic β-lactamase substrate that undergoes a distinctive color change from yellow to red as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics[1][2][3].

Product Specifications

CAS Number

[41906-86-9]

UNSPSC

12352005

Target

Antibiotic; Beta-lactamase; Reference Standards

Type

Reference Standards

Related Pathways

Anti-infection; Others

Field of Research

Infection

Assay Protocol

https://www.medchemexpress.com/nitrocefin-standard.html

Smiles

O=C(C(N12)=C(/C=C/C3=CC=C([N+]([O-])=O)C=C3[N+]([O-])=O)CS[C@]2([H])[C@H](NC(CC4=CC=CS4)=O)C1=O)O

Molecular Formula

C21H16N4O8S2

Molecular Weight

516.50

References & Citations

[1]Lee M, et al. A practical synthesis of nitrocefin. J Org Chem. 2005 Jan 7;70 (1) :367-9.|[2]Worthington RJ, et al. Overcoming resistance to β-lactam antibiotics. J Org Chem. 2013 May 3;78 (9) :4207-13.|[3]O'Callaghan CH, et al. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1 (4) :283-8.