RBC Lysis Buffer 10X (Biotechnology Grade)

<strong>RBC Lysis Buffer 10X</strong>_x000D_ <table width="1028">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="285">Catalog number:</td>_x000D_ <td width="743">B2010090</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Volume/Weight</td>_x000D_ <td>50 mL</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>pH</td>_x000D_ <td>N/A</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Supplied as</td>_x000D_ <td>10X</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Appearance</td>_x000D_ <td>Clear solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Applications</td>_x000D_ <td>This buffer is optimized for the lysis of Red Blood Cells (RBC, erythrocytes) as found in the spleen or human peripheral blood. Ammonium chloride-based, it has minimal effect on lymphocytes and nucleated RBC.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Shelf-Life</td>_x000D_ <td>1 year from date of manufacture</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>Keep at 2-8°C</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Keywords:</td>_x000D_ <td>RBC lysis, erythrocytes lysis buffer, red blood cells lysis buffer, rbc lysis buffer protocol</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Grade</td>_x000D_ <td>Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>References</td>_x000D_ <td width="743">1: Buoro S, Vavassori M, Pipitone S, Benegiamo A, Lochis E, Fumagalli S, Falanga_x000D_ A, Marchetti M, Crippa A, Ottomano C, Lippi G. Evaluation of nucleated red blood_x000D_ cell count by Sysmex XE-2100 in patients with thalassaemia or sickle cell_x000D_ anaemia and in neonates. Blood Transfus. 2015 Oct;13(4):588-94.2: Rosse WF, Dourmashkin R, Humphrey JH. Immune lysis of normal human and_x000D_ paroxysmal nocturnal hemoglobinuria (PNH) red blood cells. 3. The membrane_x000D_ defects caused by complement lysis. J Exp Med. 1966 Jun 1;123(6):969-84.3: Mauritz JM, Esposito A, Ginsburg H, Kaminski CF, Tiffert T, Lew VL. The_x000D_ homeostasis of Plasmodium falciparum-infected red blood cells. PLoS Comput Biol._x000D_ 2009 Apr;5(4):e1000339.4: Grisendi G, Finetti E, Manganaro D, Cordova N, Montagnani G, Spano C, Prapa_x000D_ M, Guarneri V, Otsuru S, Horwitz EM, Mari G, Dominici M. Detection of_x000D_ microparticles from human red blood cells by multiparametric flow cytometry._x000D_ Blood Transfus. 2015 Apr;13(2):274-80._x000D_ _x000D_ 5: van der Spuy WJ, Pretorius E. Interaction of red blood cells adjacent to and_x000D_ within a thrombus in experimental cerebral ischaemia. Thromb Res._x000D_ 2013;132(6):718-23._x000D_ 6: Dalibalta S, Ellory JC, Browning JA, Wilkins RJ, Rees DC, Gibson JS. Novel_x000D_ permeability characteristics of red blood cells from sickle cell patients_x000D_ heterozygous for HbS and HbC (HbSC genotype). Blood Cells Mol Dis. 2010 Jun_x000D_ 15;45(1):46-52._x000D_ 7: Górnicki A. The hemolysis kinetics of psoriatic red blood cells. Blood Cells_x000D_ Mol Dis. 2008 Sep-Oct;41(2):154-7._x000D_ 8: Chow S, Hedley D, Grom P, Magari R, Jacobberger JW, Shankey TV. Whole blood_x000D_ fixation and permeabilization protocol with red blood cell lysis for flow cytometry of intracellular phosphorylated epitopes in leukocyte subpopulations._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/16080188/">Cytometry</a> A. 2005 Sep;67(1):4-17.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Related Products</td>_x000D_ <td><a href="https://moleculardepot.com/product-category/buffers/">Biochemical Buffers and Solutions</a></td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>