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3C Protease

3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.

Product Specifications

Expression System

E.coli

Nature

Recombinant

Applications

Remove fusion tags from proteins with the HRV 3C cleavage sequence (LEVLFQ↓GP) .

Stability

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Endotoxin

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Bioactivity

One unit will cleave ≥95% of 100μg of GST-tagged protein in 16 hours at 4°C.

Form

Liquid

Buffer

50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol

Shipping Conditions

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Notes

For research use only.

Species

HRV-14

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