Anti-STAT5A (phospho-Tyr694) Antibody

Rabbit Polyclonal Antibody specific to STAT5A (phospho-Tyr694)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-STAT5A (phospho-Tyr694) Rabbit Polyclonal Antibody

Gene Name

STAT5A

Gene ID

601511

NCBI Gene ID

UniProt

P42229

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of tyrosine 694 (D-G-Y(p)-V-K) derived from human STAT5A and conjugated to KLH.

Target Antigen

Signal transducer and activator of transcription 5A

Target

STAT5A (phospho-Tyr694)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. {PubMed:15534001}.

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of~90kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat STAT5A only when phosphorylated at tyrosine 694.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Signal transducers and activators of transcription (STATs) are transcription factors that play important roles in signaling pathways induced by many different cytokines and growth factors that regulate cell growth and differentiation, the immune response, antiviral activity, and homeostasis. Their activation and mechanisms of action are examples of direct signaling to the nucleus by extracellular polypeptide ligands without the involvement of second messengers, tyrosine phosphorylation of transcription factors, or transcription factors that make direct contact with membrane-bound receptors and DNA. Since the discovery of STAT 1 and 2, the family has been expanded to seven members.