Anti-PINK1 Antibody

Mouse Monoclonal Antibody specific to PINK1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-PINK1 Mouse Monoclonal Antibody

Gene Name

PINK1

NCBI Gene ID

UniProt

Q9BXM7.1

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 112-496 (cytoplasmic C-terminus) of human PINK1. This sequence is 82

Target Antigen

Serine/threonine-protein kinase PINK1, mitochondrial

Target

PINK1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~50kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat PINK1.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

PINK1 (PTEN-induced putative kinase 1) is a mitochondrial serine/threonine kinase that maintains mitochondrial function and integrity, provides protection against mitochondrial dysfunction during cellular stress, and is involved in the clearance of damaged mitochondria via selective autophagy. PINK1 is synthesized as a 63kDa protein which undergoes proteolytic processing to yield two cleaved forms, ~55kDa and ~42kDa. PINK1 may be localized to the outer mitochondrial membrane with its kinase domain facing the cytosol which would explain the observed physical interaction between PINK1 and cytosolic E3 ubiquitin ligase Parkin.