BODIPY FL-EDA

BODIPY FL-EDA is a widely used fluorescent dye for quantitative analysis of nucleotides. BODIPY FL-EDA is an aliphatic amine analog that can react with aldehydes and ketones. BODIPY FL-EDA can be used to detect both modified and unmodified deoxynucleotides and to determine DNA damage and genomic DNA methylation through capillary electrophoresis with laser-induced fluorescence (CE-LIF) . Additionally, it can be used for quantifying intracellular ATP levels. The excitation wavelength is 500 nm, and the emission wavelength is 510 nm[1][2][3].

Product Specifications

CAS Number

[209541-26-4]

UNSPSC

12171500

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/bodipy-fl-eda.html

Purity

99.23

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

[F-][B+3]1([N-]2C(C=C(C(C)=C3)[N]1=C3C)=CC=C2CCC(NCCN)=O)[F-].Cl

Molecular Formula

C16H22BClF2N4O

Molecular Weight

370.63

References & Citations

[1]Cornelius M, et al. Detection and separation of nucleoside-5'-monophosphates of DNA by conjugation with the fluorescent dye BODIPY and capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis. 2005 Jun;26 (13) :2591-8. |[2]Zinellu A, et al. Capillary electrophoresis with laser-induced fluorescence detection for ATP quantification in spermatozoa and oocytes. Anal Bioanal Chem. 2010 Nov;398 (5) :2109-16.|[3]Cornelius MG, et al. RNA analysis by MEKC with LIF detection. Electrophoresis. 2007 Nov;28 (21) :3901-7.