Anti-p95/Nibrin Antibody
Mouse Monoclonal Antibody specific to p95
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1
Gene Name
NBN
NCBI Gene ID
<a href="https://www.ncbi.nlm.nih.gov/gene/?term=NBN">NBN</a>
UniProt
O60934
Cellular Locus
Nucleus, Nucleus, PML body, Chromosome, telomere, Chromosome
Host
Mouse
Reactivity
Human
Immunogen
Fusion protein containing the complete coding region of human p95/nibrin expressed in E. coli.
Target Antigen
Nibrin
Target
p95/Nibrin
Clonality
Monoclonal
Isotype
IgG1
Type
Antibody
Applications
WB, ICC/IF, IP
Field of Research
Cancer research
Purification Method
Purified by Protein G affinity chromatography
Concentration
Lot Specific
Dilution
Dilute in PBS or medium which is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Function
Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:15616588, PubMed:19759395, PubMed:23762398, PubMed:26438602, PubMed:9705271}.
Additionnal Information
Immunoblotting,
<br><br>Immunoprecipitation:: use at 1-2 ug/mL. In immunoblots, a band of 95 kD is detected.
<br><br>Positive controls: MCF-7, HeLa, or Raji cells.
Storage Conditions
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Specificity
This antibody recognizes the 95 kD nibrin protein which contains a forkhead-associated domain that is adjacent to BRCT, a breast cancer C- terminal domain involved in protein- protein interactions. p95/nibrin is a member of the Mre11/Rad50 double- strand break repair complex.
Formulation
PBS, pH 7.4.
Buffer pH
pH 7.4
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