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Rat anti Plet-1

Product Specifications

Background

The 33A10-defined antigen is the orphan protein, Placenta expressed transcript (Plet)-1. Biochemical data indiCated that Plet-1 is a glycosylphosphatidylinositol-anchored glycoprotein with N-linked carbohydRates in addition to other posttranslational modifiCations. Plet-1 is a a recently identified protein that as of yet has no known function and is not associated with any one family of proteins. Plet-1 is used as a cell surface marker present on stem/progenitor epithelial cells and in a number of tissues including thymus and skin, and on epithelial cell types in mammary gland, prostate, and pancreas.

UniProt

Q8VEN2

Host

Rat

Species Reactivity

Mouse

Isotype

IgG2b

Clone

33A10

Type

Primary Antibodies

Source

33A10 is a Rat IgG2b monoclonal antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells of Sprague-Dawley Rats immunized with Mouse mammary tumor cells.

Applications

Flow Cytometry, IF, IHC (frozen), WB

Field of Research

Other

Assay Principle

33A10 is useful for Flow Cytometry, Immunofluorescence, Immunohistochemistry (frozen). Optimal antibody dilution should be determined by titration.

Form

Each vial contains 100 µL 1 mg/mL purified monoclonal antibody in PBS containing 0,09% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

References & Citations

1. A Sonnenberg, H Daams, M A Van der Valk, J Hilkens and J Hilgers (1986), J Histochem Cytochem 34 (8): 1037-1046. _x000D_ 2. Karine Raymond, Anja Richter, Maaike Kreft, Evelyne Frijns, Hans Janssen, Monique Slijper, Silke Praetzel-Wunder, Lutz Langbein and Arnoud Sonnenberg (2010), J Invest Derm 130 1500-1513.

Storage Conditions

The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product

CAS Number

9007-83-4

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