Acriflavine (hydrochloride)

Acriflavine (Acriflavinium chloride) hydrochloride is a fluorescent acridine dye that can be used to label nucleic acid. Acriflavine hydrochloride is an antiseptic agent. Acriflavine hydrochloride is a potent HIF-1 inhibitor that prevents the dimerization of HIF-1α and HIF-1β subunits. Acriflavine hydrochloride inhibits the interaction between monocarboxylate transporter 4 (MCT4) and Basigin. Acriflavine hydrochloride is used in cancer research, such as breast cancer, brain tumor and chronic myeloid leukemia. Acriflavine hydrochloride is a potent papain-like protease (PLpro) inhibitor, which inhibits SARS-CoV-2[1][2][3][4][5][6].

Product Specifications

CAS Number

[69235-50-3]

Product Name Alternative

Acriflavinium (chloride hydrochloride)

UNSPSC

12352005

Hazard Statement

H302, H315, H318, H411

Target

Bacterial; Fluorescent Dye; Fungal; HIF/HIF Prolyl-Hydroxylase; Monocarboxylate Transporter; SARS-CoV

Type

Reference compound

Related Pathways

Anti-infection; Membrane Transporter/Ion Channel; Metabolic Enzyme/Protease; Others

Applications

COVID-19-anti-virus

Field of Research

Cancer; Infection

Assay Protocol

https://www.medchemexpress.com/acriflavine-hydrochloride.html

Concentration

10mM

Purity

99.4

Solubility

DMSO : 25 mg/mL (ultrasonic)

Smiles

C[N+]1=C2C=C(N)C=CC2=CC3=C1C=C(N)C=C3.[H]Cl.NC4=CC=C5C(N=C6C=C(N)C=CC6=C5)=C4.[H]Cl.[Cl-]

Molecular Formula

C27H27Cl3N6

Molecular Weight

541.90

Precautions

H302, H315, H318, H411

References & Citations

[1]L M Chan, et al. Interaction of acriflavine with DNA and RNA. J Mol Biol. 1969 Mar 28;40 (3) :491-5.|[2]Thomas Meinelt, et al. Effects of Calcium Content and Humic Substances on the Toxicity of Acriflavine to Juvenile Zebrafish Danio rerio. Journal of Aquatic Animal Health, 14:1, 35-38.|[3]Tao Yin, et al. HIF-1 Dimerization Inhibitor Acriflavine Enhances Antitumor Activity of Sunitinib in Breast Cancer Model. Oncol Res. 2015; 22 (3) : 139-145.|[4]Voss DM, et al. Disruption of the monocarboxylate transporter-4-basigin interaction inhibits the hypoxic response, proliferation, and tumor progression. Sci Rep. 2017 Jun 27;7 (1) :4292.|[5]Cheloni G, et al. Targeting chronic myeloid leukemia stem cells with the hypoxia-inducible factor inhibitor acriflavine. Blood. 2017 Aug 3;130 (5) :655-665.|[6]Valeria Napolitano, et al. Acriflavine, a clinically aproved drug, inhibits SARS-CoV-2 and other betacoronaviruses. bioRxiv 2021.03.20.436259.