Anti-TrpC5 Ca+2 Channel Antibody

Mouse Monoclonal Antibody specific to TrpC5 Ca+2 Channel

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

TrpC5, Transient receptor protein 5, TRP-5, hTRP-5, hTRP5

Gene Name

TRPC5

NCBI Gene ID

UniProt

Q9UL62

Accession Number

NP_036603.1

Cellular Locus

Cell membrane, Multi-pass membrane protein

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to aa 827-845 of human TrpC5 (accession no. Q9UL62).

Target Antigen

Short transient receptor potential channel 5

Target

TrpC5 Ca+2 Channel

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, IHC, IP, AM

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol sUniProtKB:Q9QX29, PubMed:16284075}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~110kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat TrpC5.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. TrpC5 (transient receptor potential cation channel, subfamily C, member 5) is a subtype of the TrpC family of mammalian transient receptor potential ion channels. Homomultimeric TrpC5 and heteromultimeric TrpC5-TrpC1 channels are activated by extracellular reduced thioredoxin. This activation has been associated with rheumatoid arthritis as well as with action on anesthetics such as chloroform, halothane, and propofol.