Mouse anti Human Proximal Nephrogenic Renal Antigen, cln PN-15 (66.4.C2)
Selected Variant
X1234M
Size: 100 µg
Product Specifications
Background
This antibody reacts with a glycoprotein of 200 kDa present in proximal renal tubules. The antigen is a carbohydrate in nature and retained in formalin fixed paraffin embedded tissues. Other normal tissues that display the antigen include breast, parathyroid glands and epididymis. Among renal carcinomas, 93% of primary and 84% of metastatic carcinomas are positive for this antigen. Relatively few other tumor types are positive: breast cancers, teratocarcinomas and parathyroid adenomas.
CAS Number
9007-83-4
UniProt
Q16790
Host
Mouse
Species Reactivity
Human
Isotype
IgG2b
Clone
66.4.C2
Conjugation
Unconjugated
Type
Monoclonal Antibody
Source
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with renal cortical tissue extract and mouse myeloma cells
Applications
IHC (paraffin), IHC (frozen)
Field of Research
Infectious Agents and Disease, Oncology
Purification Method
Protein A/G Chromatography
Assay Principle
This antibody can be used for the differential diagnosis of carcinomas by Western blotting and immunohistochemistry on frozen and paraffin-embedded tissues. Protease digestion prior to antibody incubation enhances staining of tissue sections. Optimal concentration should be evaluated by serial dilutions.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Yoshida, S.O. and Imam, A., 'Monoclonal antibody to a proximal nephrogenic renal antigen: immunohistochemical analysis of formalin-fixed, paraffin-embedded Human renal cell carcinomas.' Cancer Res. 1989, 49, 1802-1809._x000D_
2. Piron, A., et al. 'In vitro demonstration of a mitogenic activity in renal tissue extracts during regenerative hyperplasia.' Am. J. Physiol. 1998, 274, F348-F357.
Shipping Conditions
Ambient Temperature, freeze upon arrival
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
Paraffin Section Immunostaining
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