E-64

E-64 (Proteinase inhibitor E 64) is a potent irreversible inhibitor against general cysteine proteases with IC50 of 9 nM for papain.

Product Specifications

CAS Number

[66701-25-5]

Product Name Alternative

Proteinase inhibitor E 64

UNSPSC

12352005

Hazard Statement

H372

Target

Autophagy; Bacterial; Cathepsin

Type

Reference compound

Related Pathways

Anti-infection; Autophagy; Metabolic Enzyme/Protease

Applications

COVID-19-immunoregulation

Field of Research

Inflammation/Immunology; Cancer

Assay Protocol

https://www.medchemexpress.com/E-64.html

Purity

99.96

Solubility

DMSO : 62.5 mg/mL (ultrasonic) |H2O : 5 mg/mL (ultrasonic)

Smiles

O=C([C@H]1O[C@@H]1C(N[C@H](C(NCCCCNC(N)=N)=O)CC(C)C)=O)O

Molecular Formula

C15H27N5O5

Molecular Weight

357.41

Precautions

H372

References & Citations

[1]Matsumoto K, et al. Structural basis of inhibition of cysteine proteases by E-64 and its derivatives. Biopolymers. 1999;51 (1) :99-107.|[2]Wadhawan M, et al. Inhibition of cathepsin B by E-64 induces oxidative stress and apoptosis in filarial parasite. PLoS One. 2014 Mar 25;9 (3) :e93161.|[3]Baldzizhar R, et al. Anti-serpin antibody-mediated regulation of proteases in autoimmune diabetes. J Biol Chem. 2013 Jan 18;288 (3) :1612-9.|[4]Blass G, et al. Chronic cathepsin inhibition by E-64 in Dahl salt-sensitive rats. Physiol Rep. 2016 Sep;4 (17) . pii: e12950.