Necrostatin-1

Necrostatin-1 (Nec-1) is a potent and cross the blood-brain barrier necroptosis inhibitor with an EC50 of 490 nM in Jurkat cells. Necrostatin-1 inhibits RIP1 kinase (EC50=182 nM) . Necrostatin-1 is also an IDO inhibitor[1].

Product Specifications

CAS Number

[4311-88-0]

Product Name Alternative

Nec-1

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Autophagy; Ferroptosis; Indoleamine 2,3-Dioxygenase (IDO) ; Necroptosis; RIP kinase

Type

Reference compound

Related Pathways

Apoptosis; Autophagy; Metabolic Enzyme/Protease

Applications

Cancer-Kinase/protease

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/Necrostatin-1.html

Purity

99.89

Solubility

DMSO : 125 mg/mL (ultrasonic)

Smiles

O=C(C(CC1=CNC2=C1C=CC=C2)N3)N(C)C3=S

Molecular Formula

C13H13N3OS

Molecular Weight

259.33

Precautions

H302, H315, H319, H335

References & Citations

[1]Degterev A, et al. Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat Chem Biol. 2005 Jul;1 (2) :112-9.|[2]Linkermann A, et al. The RIP1-kinase inhibitor necrostatin-1 prevents osmotic nephrosis and contrast-induced AKI in mice. J Am Soc Nephrol. 2013 Oct;24 (10) :1545-57.|[3]Huang C, et al. Shikonin kills glioma cells through necroptosis mediated by RIP-1. PLoS One. 2013 Jun 28;8 (6) :e66326.|[4]Feyen D, et al. Increasing short-term cardiomyocyte progenitor cell (CMPC) survival by necrostatin-1 did not further preserve cardiac function. Cardiovasc Res. 2013 Jul 1;99 (1) :83-91.|[5]Zhou K, et al. RIP1-RIP3-DRP1 pathway regulates NLRP3 inflammasome activation following subarachnoid hemorrhage. Exp Neurol. 2017 Sep;295:116-124.