CCCP

CCCP is an oxidative phosphorylation (OXPHOS) uncoupler. CCCP induces activation of PINK1 leading to Parkin Ser65 phosphorylation[1].

Product Specifications

CAS Number

[555-60-2]

Product Name Alternative

Carbonyl cyanide 3-chlorophenylhydrazone; Carbonyl Cyanide m-Chlorophenylhydrazone

UNSPSC

12352005

Hazard Statement

H301+H311+H331

Target

Apoptosis; Bacterial; IFNAR; Mitochondrial Metabolism; Oxidative Phosphorylation; PINK1/Parkin; STING

Type

Reference compound

Related Pathways

Anti-infection; Apoptosis; Autophagy; Immunology/Inflammation; Metabolic Enzyme/Protease; Neuronal Signaling

Applications

Cancer-Kinase/protease

Field of Research

Cancer; Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/CCCP.html

Purity

99.83

Solubility

DMSO : 50 mg/mL (ultrasonic) |H2O : < 0.1 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

N#C/C(C#N)=N/NC1=CC=CC(Cl)=C1

Molecular Formula

C9H5ClN4

Molecular Weight

204.62

Precautions

H301+H311+H331

References & Citations

[1]Kwon D, et al. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) suppresses STING-mediated DNA sensing pathway through inducing mitochondrial fission. Biochem Biophys Res Commun. 2017 Aug 30. pii: S0006-291X (17) 31704-7.|[2]Sinha D, et al. Synergistic efficacy of Bisbenzimidazole and Carbonyl Cyanide 3-Chlorophenylhydrazonecombination against MDR bacterial strains. Sci Rep. 2017 Mar 17;7:44419.|[3]Kawamoto A, et al. Measurement of technetium-99m sestamibi signals in rats administered a mitochondrial uncoupler and in a rat model of heart failure. PLoS One. 2015 Jan 16;10 (1) :e0117091.|[4]Kondapalli C, et al. PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65. Open Biol. 2012 May;2 (5) :120080.|[5]Haifeng Jiao, et al. Mitocytosis, a migrasome-mediated mitochondrial quality-control process. Cell. 2021 May 27;184 (11) :2896-2910.e13.