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Anti-6 His Antibody, FITC conjugate

Goat Polyclonal Antibody specific to 6 His FITC

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-6 His FITC Goat Polyclonal Antibody

Host

Goat

Reactivity

6 His

Immunogen

6-His (HHHHHH) conjugated to KLH.

Target

6 His

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

IHC, ICC, IF

Field of Research

Epitope Tags

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

<br><br>Immunocytochemistry: use at a dilution of 1:100-1:400. <br><br>Immunofluorescence: use at a dilution of 1:100-1:400. <br><br>These are recommended dilutions. <br><br>End users should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at 2-8°C.

Specificity

Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.

Formulation

Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide

Buffer pH

pH 7.2

Target Background

A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

Frequently Asked Questions

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