Chicken Bovine IgG (H+L), conjugated with Agarose Antibody

Chickens were immunized with highly purified bovine IgG isolated from normal pooled bovine serum. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbant and conjugated to N-hydroxysuccinimide (NHS) -activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.