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Biotinylated Human HLA-A*11:01&B2M&KRASG12S (VVVGASGVGK) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*11:01&B2M&KRASG12S (VVVGASGVGK) Complex Protein (HLS-H82E8) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGASGVGK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGASGVGK) .

Product Specifications

Specifications

Biotinylated Human HLA-A*11:01&B2M&KRASG12S (VVVGASGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12S peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGASGVGK

Expression Region

Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & VVVGASGVGK peptide

Host

HEK293

Target

HLA-A*1101 & B2M & KRASG12S (VVVGASGVGK)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human HLA-A*11:01&B2M&KRASG12S (VVVGASGVGK) Complex Protein (Cat. No. HLS-H82E8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.2-3 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

36.0 kDa and 11.7 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

25ug*1

Host or Source

HEK293

Species

Human

Protein ID

Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & VVVGASGVGK

Preservative

Trehalose

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