Recombinant Moringa oleifera Chitin-binding protein 3

Chitin-binding protein (PubMed:23193603) . Has antifungal activity against F.solani, F.oxysporum, C.musae and C.gloesporoides but not against P.oligandrum (PubMed:23193603) . Depending on concentration the antifungal activity can be fungistatic or fungicidal (PubMed:23193603) . Inhibits both spore germination and mycelial growth in F.solani at a concentration of 0.1 mg/ml (PubMed:23193603) . Has antifungal activity against C.krusei, C.albicans, C.tropicalis and C.parapsilosis (PubMed:28634471) . Has no chitinase, beta-glucanase or hemagglutinating activity (PubMed:23193603) . Acts as a flocculent (PubMed:23193603) .

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

"A Chitin-binding Protein Purified from Moringa oleifera Seeds Presents Anticandidal Activity by Increasing Cell Membrane Permeability and Reactive Oxygen Species Production." Neto J.X.S., Pereira M.L., Oliveira J.T.A., Rocha-Bezerra L.C.B., Lopes T.D.P., Costa H.P.S., Sousa D.O.B., Rocha B.A.M., Grangeiro T.B., Freire J.E.C., Monteiro-Moreira A.C.O., Lobo M.D.P., Brilhante R.S.N., Vasconcelos I.M. Front. Microbiol. 8:980-980 (2017)

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.