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Biotinylated Rhesus macaque IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc, Avitag™&Fc, Avitag™ (MALS verified)

Selected Variant

ILG-R82F3-200ug

Size: 200 µg

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit) . The high and intermediate affinity forms also associate with a gamma subunit.

Product Specifications

Background

Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit) . The high and intermediate affinity forms also associate with a gamma subunit.

Specifications

Biotinylated Rhesus macaque IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc, Avitag&Fc, Avitag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 53 kDa (IL-2RB) and 55.4 kDa (IL-2RG) . Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein migrates as 65-75 kDa and 85-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

NP_001244989.1 & Q38JL2-1

Expression Region

Ala 27 - Thr 240 & Leu 23 - Pro 255

Host

HEK293

Target

IL-2 R beta & IL-2 R gamma

Conjugation

Biotin-labeled

Tag

C-Fc & C-Avi | C-Fc & C-Avi

Source

Rhesus macaque

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human IL-2 Protein, Tag Free (Cat. No. IL2-H5215) at 5 μg/mL (100 μL/well) can bind Biotinylated Rhesus macaque IL-2 R beta&IL-2 R gamma Heterodimer Protein, Fc,Avitag&Fc,Avitag (Cat. No. ILG-R82F3) with a linear range of 0.005-0.625 μg/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

53.0 kDa & 55.4 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Rhesus macaque

Protein ID

NP_001244989.1 & Q38JL2-1

Preservative

Trehalose

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