Phospho-Histone H2A.X (Ser139) rabbit mAb Antibody
Histone H2AX is a variant of the nucleosome core histone H2A and is phosphorylated at Ser139 in response to DNA damage. Histone H2AX phosphorylation is considered a specific reporter of double-strand DNA breaks. The protein is also referred to as gH2AX when phosphorylated at Ser139. H2AX phosphorylation is especially strong in response to double-strand breaks formed during apoptosis. However, physiological phosphorylation of Histone H2AX occurs when double-strand DNA breaks are formed during meiosis and V (D) J recombination. A549 and DU145 cell lines have been found to have higher expression levels of phosphorylated Histone H2AX compared to Jurkat, MCF-7, or HL-60 cell lines.
Product Specifications
Product Name Alternative
H2AFX, H2AX, γH2AX, gamma-H2AX
UniProt
P16104
Reactivity
Human, Mouse
Cross Reactivity
Predicted to work with mouse, rat, and other homologues.
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho histone H2A.X.
Clonality
Monoclonal
Clone
B3D5
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Protein A+G
Concentration
0.5 mg/mL
Dilution
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Tested Applications
FC, WB
Host or Source
Rabbit
Preservative
1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
Isotype
Rabbit IgG1k
Available Sizes
Frequently Asked Questions
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