Anti-mGluR1/5 Glutamate Receptor Antibody

Mouse Monoclonal Antibody specific to mGluR1/5 Glutamate Receptor

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

mGluR5

Gene Name

Grm5

NCBI Gene ID

UniProt

P31424

Cellular Locus

Cell membrane, Multi-pass membrane protein

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession no. NP_058708.1).

Target Antigen

Metabotropic glutamate receptor 5

Target

mGluR1/5 Glutamate Receptor

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, IP

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium sPubMed:1320017, PubMed:21795692}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~130kDa is detected. Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Rat brain membranes.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat mGluR1/5.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

The metabotropic glutamate receptors (mGluRs) are glutamate receptors that are active through an indirect metabotropic process. They are members of the group C family of G-protein-coupled receptors (GPCRs). The mGluRs perform a variety of functions in the central and peripheral nervous systems. They are found in pre- and postsynaptic neurons in synapses of the hippocampus, cerebellum, and the cerebral cortex, as well as other parts of the brain and in peripheral tissues. Unlike ionotropic receptors, metabotropic glutamate receptors are not ion channels. Instead, they activate biochemical cascades, leading to the modification of other proteins.