Anti-Beta 3 GABA-A Receptor Antibody

Mouse Monoclonal Antibody specific to Beta 3 GABA-A Receptor

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GABA(A receptor subunit beta-3

Gene Name

Gabrb3

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Gabrb3">Gabrb3</a>

UniProt

P63080

Cellular Locus

Cell junction, synapse, postsynaptic cell membrane, Cell membrane, Cytoplasmic vesicle membrane

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 370-433 of mouse Beta 3 GABA-A receptor (accession no. AAB60502).

Target Antigen

γ-aminobutyric acid receptor subunit beta-3

Target

Beta 3 GABA-A Receptor

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, AM

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:9108119). Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA- gated ion channel (PubMed:27129275). The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer (PubMed:27129275). The alpha1/beta3/gamma2 receptor exhibits synatogenic activity whereas the alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity (PubMed:27129275). Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). Plays an important role in somatosensation and in the production of antinociception (PubMed:10670447). {UniProtKB:P28472, PubMed:10670447, PubMed:27129275, PubMed:9108119}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~55kDa is detected. Immunohistochemistry: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Beta 3 GABA-A receptor. It does not cross-react with Beta 1 or 2 GABA-A receptor.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins thathelp establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. In some ion channels, the flow of ions is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. The GABA-A receptor is a fast acting ligand-gated ion channel. GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain.