Anti-E-Cadherin Antibody

Mouse Monoclonal Antibody specific to Human E-cadherin

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD antigen CD324 [Cleaved into: E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3]

Gene Name

CDH1

NCBI Gene ID

UniProt

P12830

Cellular Locus

Cell junction, adherens junction, Cell membrane, Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with α-, beta- and γ-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

Host

Mouse

Reactivity

Human

Immunogen

Human placenta E-Cadherin

Target Antigen

Cadherin-1

Target

E-Cadherin

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, ELISA, IHC, IF, FACS

Field of Research

Cell adhesion

Purification Method

Purified by immunoaffinity chromatography

Dilution

Reconstitute lyophilized antibody in 100ul of distilled water (final concentration 2.0mg/ml).

Format

Purified

Form

Lyophilized

Buffer

Phosphate Buffered Saline

Function

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. {PubMed:11976333, PubMed:16417575}.; E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. {PubMed:16417575}.; (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. {PubMed:10406800, PubMed:17540170, PubMed:8601315}.

Additionnal Information

ELISA: 2ug/ml Western Blotting Flow : 2ug/ml, reducing and non-reducing conditions Flow Cytometry: 1ug/ml. Immunohistochemistry: 2ug/ml, paraffin- embedded and frozen tissue sections. Immunofluorescence: 1ug/ml.Cell-Cell Contact Inhibition Assays: 1ug/ml.

Storage Conditions

Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution in aliquots at -20°C for one year or at 4°C for 6 months after addition of 0.1% sodium azide by the user. Avoid repeated freeze-thaw cycles.

Specificity

This antibody specifically reacts with human E-Cadherin, but does not react with rat E-Cadherin. This antibody inhibits E-Cadherin dependent cell-cell contact.