Mouse anti-TNF alpha Antibody
Product Specifications
Background
Mouse monoclonal antibody to TNF alpha
Synonyms
TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human TNF alpha. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of TNF alpha expression in recombinant human TNF-a protein (A) whole cell lysates.
IHC description
Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
7124
SwissProt (Human)
P01375
CAS Number
9007-83-4
Available Sizes
Curated Selection
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TA500379BM 100 µL
SIGLEC9 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1D9]
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