Mouse anti-PCNA Antibody
Product Specifications
Background
Mouse monoclonal antibody to PCNA
Synonyms
Proliferating cell nuclear antigen; PCNA; Cyclin
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human PCNA. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/2000 - 1/5000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PCNA expression in Hela (A), 293T (B), NIH3T3 (C), rat brain (D) whole cell lysates.
IHC description
Immunohistochemical analysis of PCNA staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5111
Gene ID (Mouse)
18538
Gene ID (Rat)
25737
SwissProt (Human)
P12004
SwissProt (Mouse)
P17918
SwissProt (Rat)
P04961
CAS Number
9007-83-4
Available Sizes
Curated Selection
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