Mouse anti-NF-kappaB p65 Antibody

Product Specifications

Background

Mouse monoclonal antibody to NF-kappaB p65

CAS Number

9007-83-4

Synonyms

NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Host

Mouse

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human NF-kappaB p65. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IP

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/3000), IH (1/100 - 1/200), IP (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of NF-kappaB p65 expression in Hela (A), 293T (B), HepG (C) whole cell lysates.

IHC description

Immunohistochemical analysis of NF-kappaB p65 staining in mouse hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.