Mouse anti-HSP90 alpha Antibody

Product Specifications

Background

Mouse monoclonal antibody to HSP90 alpha

CAS Number

9007-83-4

Synonyms

HSP90A; HSPC1; HSPCA; Heat shock protein HSP 90-alpha; Heat shock 86 kDa; HSP 86; HSP86; Lipopolysaccharide-associated protein 2; LAP-2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38

Host

Mouse

Reactivity

H, M, R

Immunogen

Recombinant protein corresponding to human HSP90 alpha.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/2000 - 1/5000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of HSP90 alpha expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of HSP90 alpha staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.