Mouse anti-HSP27 Antibody
Product Specifications
Background
Mouse monoclonal antibody to HSP27
Synonyms
HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27
Host
Mouse
Reactivity
H
Immunogen
Recombinant protein corresponding to human HSP27.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HSP27 expression in Hela (A) whole cell lysates.
IHC description
Immunohistochemical analysis of HSP27 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3315
SwissProt (Human)
P04792
CAS Number
9007-83-4
Available Sizes
Curated Selection
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