Mouse anti-HSC70 Antibody

Product Specifications

Background

Mouse monoclonal antibody to HSC70

CAS Number

9007-83-4

Synonyms

HSC70; HSP73; HSPA10; Heat shock cognate 71 kDa protein; Heat shock 70 kDa protein 8; Lipopolysaccharide-associated protein 1; LAP-1; LPS-associated protein 1

Host

Mouse

Reactivity

H, M, R

Immunogen

Recombinant protein corresponding to human HSC70.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/2000 - 1/5000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of HSC70 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of HSC70 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.