Mouse anti-Histone H2B Antibody
Product Specifications
Background
Mouse monoclonal antibody to Histone H2B
CAS Number
9007-83-4
Synonyms
H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Histone H2B. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1mg/mL
Dilution
WB (1/1000 - 1/3000), IH (1/200 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Mouse
WB Description
Western blot analysis of Histone H2B expression in Hela (A), NIH3T3 (B), Raw264.7 (C), rat brain (D), rat kidney (E) whole cell lysates.
IHC description
Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3018
Gene ID (Mouse)
319178
SwissProt (Human)
P33778
SwissProt (Mouse)
Q64475
Available Sizes
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