Mouse anti-GFAP Antibody
Product Specifications
Background
Mouse monoclonal antibody to GFAP
Synonyms
Glial fibrillary acidic protein; GFAP
Host
Mouse
Reactivity
M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of mouse GFAP. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/2000 - 1/5000), IH (1/200 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GFAP expression in mouse brain (A), rat brain (B) whole cell lysates.
IHC description
Immunohistochemical analysis of GFAP staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Mouse)
14580
Gene ID (Rat)
24387
SwissProt (Mouse)
P03995
SwissProt (Rat)
P47819
CAS Number
9007-83-4
Available Sizes
Curated Selection
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