Mouse anti-Gamma-enolase Antibody
Product Specifications
Background
Mouse monoclonal antibody to Gamma-enolase
Synonyms
Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Gamma-enolase. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Gamma-enolase expression in Hela (A), Jurkat (B), 293T (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Gamma-enolase staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
2026
Gene ID (Mouse)
13807
Gene ID (Rat)
100911625
SwissProt (Human)
P09104
SwissProt (Mouse)
P17183
SwissProt (Rat)
P07323
CAS Number
9007-83-4
Available Sizes
Curated Selection
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