What is the catalog number for Mouse anti-eIF4AI Antibody?

The catalog number for Mouse anti-eIF4AI Antibody is YLD8437.

What sizes are available for Mouse anti-eIF4AI Antibody?

Mouse anti-eIF4AI Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Mouse anti-eIF4AI Antibody come in?

Mouse anti-eIF4AI Antibody is available in 2 different pack sizes.

Is Mouse anti-eIF4AI Antibody in stock?

Mouse anti-eIF4AI Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Mouse anti-eIF4AI Antibody

Product Specifications

Background

Mouse monoclonal antibody to eIF4AI

CAS Number

9007-83-4

Synonyms

DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1

Host

Mouse

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human eIF4AI. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IF/IC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/3000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of eIF4AI expression in 293T (A), Hela (B), HepG2 (C), mouse brain (D) whole cell lysates.

IHC description

Immunohistochemical analysis of eIF4AI staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of eIF4AI staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .