Mouse anti-Cytokeratin 8 Antibody
Product Specifications
Background
Mouse monoclonal antibody to Cytokeratin 8
Synonyms
CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Cytokeratin 8. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/2000 - 1/5000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Cytokeratin 8 expression in Hela (A), mouse brain (B) whole cell lysates.
IHC description
Immunohistochemical analysis of Cytokeratin 8 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3856
Gene ID (Mouse)
16691
Gene ID (Rat)
25626
SwissProt (Human)
P05787
SwissProt (Mouse)
P11679
SwissProt (Rat)
Q10758
CAS Number
9007-83-4
Available Sizes
Curated Selection
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