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Mouse anti-Cytokeratin 7 Antibody

Product Specifications

Background

Mouse monoclonal antibody to Cytokeratin 7

Synonyms

SCL; Keratin type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7

Host

Mouse

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human Cytokeratin 7. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IP

Concentration

1 mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/100 - 1/200), IP (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Cytokeratin 7 expression in Hela (A), mouse kidney (B), mouse brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Cytokeratin 7 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description

Immunoprecipitation of Cytokeratin 7 from 0.5mg Hela whole cell extract lysate, using anti-Cytokeratin 7 Antibody.

Gene ID (Human)

3855

Gene ID (Mouse)

110310

Gene ID (Rat)

300242

SwissProt (Human)

P08729

SwissProt (Mouse)

Q9DCV7

SwissProt (Rat)

Q6IG12

CAS Number

9007-83-4

Available Sizes

Curated Selection

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