Mouse anti-Cytokeratin 18 Antibody

Product Specifications

Background

Mouse monoclonal antibody to Cytokeratin 18

CAS Number

9007-83-4

Synonyms

CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Host

Mouse

Reactivity

H, M, R

Immunogen

Recombinant protein corresponding to human Cytokeratin 18.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/3000), IH (1/200 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of Cytokeratin 18 expression in HepG2 (A), Hela (B), mouse liver (C), mouse skeletal muscle (D), C2C12 (E), rat heart (F) whole cell lysates.

IHC description

Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.