Mouse anti-Cytokeratin 18 Antibody
Product Specifications
Background
Mouse monoclonal antibody to Cytokeratin 18
Synonyms
CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Host
Mouse
Reactivity
H, M, R
Immunogen
Recombinant protein corresponding to human Cytokeratin 18.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/3000), IH (1/200 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Cytokeratin 18 expression in HepG2 (A), Hela (B), mouse liver (C), mouse skeletal muscle (D), C2C12 (E), rat heart (F) whole cell lysates.
IHC description
Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3875
Gene ID (Mouse)
16668
Gene ID (Rat)
294853
SwissProt (Human)
P05783
SwissProt (Mouse)
P05784
SwissProt (Rat)
Q5BJY9
CAS Number
9007-83-4
Available Sizes
Curated Selection
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