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Mouse anti-COX4-1 Antibody

Product Specifications

Background

Mouse monoclonal antibody to COX4-1

CAS Number

9007-83-4

Synonyms

COX4; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1

Host

Mouse

Reactivity

H, M, R

Immunogen

Recombinant protein corresponding to human COX4-1.

Clonality

Monoclonal

Applications

WB, IF/IC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of COX4-1 expression in Hela (A), HepG2 (B), 293T (C), mouse heart (D), mouse muscle (E), rat heart (F) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of COX4-1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

Gene ID (Human)

1327

Gene ID (Mouse)

12857

Gene ID (Rat)

29445

SwissProt (Human)

P13073

SwissProt (Mouse)

P19783

SwissProt (Rat)

P10888

Available Sizes

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