Mouse anti-COX4-1 Antibody
Product Specifications
Background
Mouse monoclonal antibody to COX4-1
CAS Number
9007-83-4
Synonyms
COX4; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1
Host
Mouse
Reactivity
H, M, R
Immunogen
Recombinant protein corresponding to human COX4-1.
Clonality
Monoclonal
Applications
WB, IF/IC
Concentration
1mg/mL
Dilution
WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
Host or Source
Mouse
WB Description
Western blot analysis of COX4-1 expression in Hela (A), HepG2 (B), 293T (C), mouse heart (D), mouse muscle (E), rat heart (F) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of COX4-1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
1327
Gene ID (Mouse)
12857
Gene ID (Rat)
29445
SwissProt (Human)
P13073
SwissProt (Mouse)
P19783
SwissProt (Rat)
P10888
Available Sizes
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