Mouse anti-CD16 Antibody

Product Specifications

Background

Mouse monoclonal antibody to CD16

CAS Number

9007-83-4

Synonyms

FCGR3A; CD16A; FCG3; FCGR3; IGFR3; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD16a; FCGR3B; CD16B; FCG3; FCGR3; IGFR3; Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; Fc-gamma RIII; Fc-gamma RIIIb; FcRIII; FcRIIIb; FcR-10; IgG Fc receptor III-1; CD16b

Host

Mouse

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human CD16. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of CD16 expression in Jurkat (A), K562 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of CD16 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.