Mouse anti-Caspase 9 Antibody

Product Specifications

Background

Mouse monoclonal antibody to Caspase 9

CAS Number

9007-83-4

Synonyms

MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6

Host

Mouse

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human Caspase 9. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IF/IC, IP

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/3000), IP (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of Caspase 9 expression in Hela (A), Jurkat (B), HepG2 (C), mouse heart (D), rat heart (E) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of Caspase 9 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .

IP description

Immunoprecipitation of Caspase 9 from 0.5mg Hela whole cell extract lysate, using anti-Caspase 9 Antibody.

Gene ID (Human)

842

SwissProt (Human)

P55211

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Mouse anti-Caspase 9 Antibody

Product Specifications

Background

CAS Number

Synonyms

Host

Reactivity

Immunogen

Clonality

Applications

Concentration

Dilution

Buffer

Storage Conditions

Host or Source

WB Description

IF/ICC description

IP description

Gene ID (Human)

SwissProt (Human)

Available Sizes

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