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Mouse anti-Carbonic Anhydrase 9 Antibody Antibody

Product Specifications

Background

Mouse monoclonal antibody to Carbonic Anhydrase 9

CAS Number

9007-83-4

Synonyms

G250; MN; Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1

Host

Mouse

Reactivity

H

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human Carbonic Anhydrase 9. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IP

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/3000), IH (1/100 - 1/200), IP (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of Carbonic Anhydrase 9 expression in Hela (A), 293T (B) whole cell lysates.

IHC description

Immunohistochemical analysis of Carbonic Anhydrase 9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description

Immunoprecipitation of Carbonic Anhydrase 9 from 0.5mg Hela whole cell extract lysate, using anti-Carbonic Anhydrase 9 Antibody Antibody.

Gene ID (Human)

768

SwissProt (Human)

Q16790

Available Sizes

Frequently Asked Questions

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