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Mouse anti-Beta-tubulin Antibody

Product Specifications

Background

Mouse monoclonal antibody to Beta-tubulin

Synonyms

TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III

Host

Mouse

Reactivity

H, M, R, Mk, D, C, Rb

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IP

Concentration

1 mg/mL

Dilution

WB (1/1000 - 1/3000), IH (1/50 - 1/100), IP (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,0.2% BSA, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Beta-tubulin expression in Hela (A), mouse kidney (B), rat heart (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Beta-tubulin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description

Immunoprecipitation of Beta-tubulin from 0.5mg mouse brain whole cell extract lysate, using anti-Beta-tubulin Antibody.

Gene ID (Human)

10381

Gene ID (Mouse)

22152

Gene ID (Rat)

246118

SwissProt (Human)

Q13509

SwissProt (Mouse)

Q9ERD7

SwissProt (Rat)

Q4QRB4

CAS Number

9007-83-4

Available Sizes

Curated Selection

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