Mouse anti-Beta-catenin Antibody
Product Specifications
Background
Mouse monoclonal antibody to Beta-catenin
Synonyms
CTNNB; Catenin beta-1; Beta-catenin
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Beta-catenin. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Beta-catenin expression in Hela (A), 293T (B), MCF7 (C), mouse brain (D), rat liver (E) whole cell lysates.
IHC description
Immunohistochemical analysis of Beta-catenin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1499
Gene ID (Mouse)
12387
Gene ID (Rat)
84353
SwissProt (Human)
P35222
SwissProt (Mouse)
Q02248
SwissProt (Rat)
Q9WU82
CAS Number
9007-83-4
Available Sizes
Curated Selection
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