Mouse anti-Beta-actin Antibody
Product Specifications
Background
Mouse monoclonal antibody to Beta-actin
Synonyms
Actin cytoplasmic 1; Beta-actin
Host
Mouse
Reactivity
H, M, R, Mk, D, C, Rb
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Beta-actin. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC, IF/IC
Concentration
1 mg/mL
Dilution
WB (1/2000 - 1/5000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Beta-actin expression in 293T (A), Hela (B), mouse kidney (C), rat heart (D), rat brain (E) whole cell lysates.
IHC description
Immunohistochemical analysis of Beta-actin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Beta-actin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
60
Gene ID (Mouse)
11461
Gene ID (Rat)
81822
SwissProt (Human)
P60709
SwissProt (Mouse)
P60710
SwissProt (Rat)
P60711
CAS Number
9007-83-4
Available Sizes
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