Mouse anti-Beta-actin Antibody

Product Specifications

Background

Mouse monoclonal antibody to Beta-actin

CAS Number

9007-83-4

Synonyms

Actin cytoplasmic 1; Beta-actin

Host

Mouse

Reactivity

H, M, R, Mk, D, C, Rb

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human Beta-actin. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC, IF/IC

Concentration

1mg/mL

Dilution

WB (1/2000 - 1/5000), IH (1/100 - 1/200), IF/IC (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Mouse

WB Description

Western blot analysis of Beta-actin expression in 293T (A), Hela (B), mouse kidney (C), rat heart (D), rat brain (E) whole cell lysates.

IHC description

Immunohistochemical analysis of Beta-actin staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of Beta-actin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .