Mouse anti-BCL2 Antibody
Product Specifications
Background
Mouse monoclonal antibody to BCL2
Synonyms
Apoptosis regulator Bcl-2
Host
Mouse
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human BCL2. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IHC, IF/IC
Concentration
1 mg/mL
Dilution
WB (1/1000 - 1/2000), IH (1/200 - 1/500), IF/IC (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of BCL2 expression in Hela (A) whole cell lysates.
IHC description
Immunohistochemical analysis of BCL2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of BCL2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
596
Gene ID (Mouse)
12043
Gene ID (Rat)
24224
SwissProt (Human)
P10415
SwissProt (Mouse)
P10417
SwissProt (Rat)
P49950
CAS Number
9007-83-4
Available Sizes
Curated Selection
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